Fig. 9

Effects of conditional media (CM) from control- or SBD-treated PCa cells on tube formation, viability of HUVEC. a PCa cells were treated without or with SBD at the indicated doses for 48 h. The conditional media (CM) was then harvested and applied to HUVEC cultured in Matrigel-coated plates for an additional 4 h. Changes in cell morphology were captured using an microscope. b HUVECs were treated without or with SBD for 4 h, followed by measurement of cell viability by CCK8 (c) PCa cells were treated without or with PMA (10 nM), in the absence or presence of SBD for 4 h. The CM was then collected and applied to HUVEC changes in HUVEC tube formation were captured using an inverted microscope. Date was mean ± SEM (n = 5), p < 0.05 (*)