Fig. 3

SBD inhibited PCa cell apoptosis through Akt/PI3K signalling pathway. Cell apoptosis measured by flow cytometry after SBD treatment with different concentrations for 48 h in PC-3 (a) and DU145 (b) cell lines; Protein expression levels of Cleaved caspase-3, Bcl-2 and Bax in PC-3 and DU145 cells determined by western blotting assay; (c and d) Relative protein expression levels of p-Akt, Akt, p-PI3K and PI3K with or without SBD treatment determined by western blotting assay. Date was mean ± SEM, p < 0.05 (*)