Fig. 2

SBD inhibits the proliferation of PCa cells. Cell viabilities were measured by CCK-8 assay after treatment with indicated concentrations of SBD for 24 h (a) and 48 h (b). The cell proliferation and apoptosis of PC-3 (c) and DU145 (d) cell lines with or without SBD treatment determine by EdU and Hoechst staining. Cell cycle of PC-3 (e) and DU145 (f) cell lines measured by flow cytometry analysis after SBD treatment for 48 h. The colony formation of PC-3 (g) and DU145 (h) cell lines with or without SBD treatment. Date was mean ± SEM (n = 4), p < 0.05 (*)