Fig. 1

HF inhibits cell proliferation and induces G₂/M phase arrest in CML cells. A The chemical structure of Hinokiflavone (C₃₀H₁₈O₁₀). B The cell viability of K562 cells treated with HF (0.0, 2.5, 5.0, 10.0, 20.0, and 40.0 μM) for 24, 48 and 72 h, respectively. C The IC₅₀ values of HF on K562 cells. D The morphology changes of cells treated with HF for 48 h were observed by optical microscopy (× 200, scale bar = 50 μm). E Cell cycle distribution of K562 cells treated with HF (0, 5, 10 and 20 μM) for 48 h. F Cell cycle distribution of K562 cells treated with 10 μM HF at different time (0, 6, 12, 24, 36 and 48 h). G, H The expression levels of p21, Cdc2 and Cyclin B1 were analyzed by western blotting after treatment with different concentrations of HF (0, 5, 10 and 20 μM) and different times of 10 μM HF. *P < 0.05, **P < 0.01, ***P < 0.001 vs the control group