Hesperidin identified from Citrus extracts potently inhibits HCV genotype 3a NS3 protease

Table 2 Inhibitory effect of Citrus extracts on the activity of NS3-NS4A. The extracts were screened using the validated FRET assay, percentage inhibition and IC₅₀ values were calculated for each extract. The pomegranate pericarp extract was used as a positive control

Sr. #	Scientific name	Common name	Plant parts used	% Inhibition (\(\frac{{\varvec{a}}-{\varvec{b}}}{{\varvec{a}}}\)*100)	IC₅₀ value (µg/mL)
1	Citrus paradisi	Grapefruit	Mesocarp	91%	5.79 ± 1.44
2	Citrus sinesis	Orange	Fruit	86%	37.19 ± 5.92
3	Citrus aurantinum	Bitter Orange	Fruit	85%	42.62 ± 6.89
4	Citrus reticulata	Mandarin	Fruit	82%	57.65 ± 3.81
5	Citrus limon	Lemon	Fruit	80%	66.83 ± 4.92
6	Citrus aurantinum	Bitter Orange	Pericarp	79%	71.94 ± 2.39
7	Citrus aurantinum	Bitter Orange	Mesocarp	78%	77.06 ± 4.26
8	Citrus reticulate	Mandarin	Seeds	77%	80.02 ± 3.67
9	Citrus sinesis	Orange	Seeds	75%	86.93 ± 5.16
10	Citrus reticulate	Mandarin	Mesocarp	74%	90.59 ± 1.89
11	Citrus paradisi	Grapefruit	Pericarp	71%	107.90 ± 5.64
12	Citrus paradisi	Grapefruit	Fruit	69%	120.30 ± 2.08
13	Citrus paradisi	Grapefruit	Seeds	68%	132.23 ± 2.12
14	Citrus aurantinum	Bitter Orange	Seeds	63%	196.40 ± 2.92
15	Punica granatum	Pomegranate	Pericarp	98%	5.52 ± 0.74

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