Fig. 2From: Hesperidin identified from Citrus extracts potently inhibits HCV genotype 3a NS3 proteaseActivity analysis of purified NS3-NS4A. A The activity of NS3-NS4A increased upon the addition of the depsipeptide substrate, indicated by a corresponding increase in the fluorescence intensity. B Kinetic parameters (Km, kcat, and kcat/Km) calculated for NS3-NS4A using the Michaelis Menten equation are presentedBack to article page