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Fig. 4 | BMC Complementary Medicine and Therapies

Fig. 4

From: Inhibitory effects of Syzygium jambos extract on biomarkers of endothelial cell activation

Fig. 4

S. jambos extract downregulates the ET-1 stimulated IL-6 expression and secretion in endothelial cells. Human endothelial cells were incubated for 24 (A, C) or 48 h (B, D) in the absence or presence of 100 nM ET-1 with or without S. jambos extract (50 μg/ml). mRNA levels are presented as the fold difference in RNA level relative to unstimulated sample values using GAPDH as a control. A-B mRNA levels of IL-6 by real-time PCR was measured as described in Methods at 24 h (A) #p < 0.0002 vehicle vs ET-1; ***p < 0.0008, ET-1 vs ET-1 + S. jambos and 48 h (B) #p < 0.0003 vehicle vs ET-1; ***p < 0.0001, ET-1 vs ET-1 + S. jambos. Values represent mean ± SE (n = 6). C-D Supernatants were collected to determine secretion levels of IL-6 as measured by ELISA assay as described in Methods. Results represent the mean ± SD of n = 3. **, p < 0.01 ET-1 treated samples in absence vs presence of S. jambos extract; #p < 0.001 vehicle vs ET-1

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