Table 1 Sequences of primers used in this study.
Gene Name | Sequence |
|---|---|
PI3K | Forward: 5’-CTGCCTGCGACAGATGAGTG-3’ Reverse: 5’-TCCGATTACCAAGTGCTCTTTC-3’ |
Akt | Forward: 5’-AGCGACGTGGCTATTGTGAAG-3’ Reverse: 5’-GCCATCATTCTTGAGGAGGAAGT-3’ |
mTOR | Forward: 5’-ACAACTTTGGTATCGTGGAAGG-3’ Reverse: 5’-GCCATCACGCCACAGTTTC-3’ |
Bcl-2 | Forward: 5’-ATGTGTGTGGAGAGCGTCAACC-3’ Reverse: 5’-CAGAGACAGCCAGGAGAAATCAA-3’ |
Caspase-9 | Forward: 5’-CTCAGACCAGAGATTCGCAAAC-3’ Reverse: 5’-GCATTTCCCCTCAAACTCTCAA-3’ |
NF-κB | Forward: 5’-GAAGCACGAATGACAGAGGC-3’ Reverse: 5’-GCTTGGCGGATTAGCTCTTTT-3’ |
VEGF | Forward: 5’-TGCCCACTGAGGAGTCCAAC-3’ Reverse: 5’-TGGTTCCCGAAACGCTGAG-3’ |
HIF-1α | Forward: 5’-ATTCCAGCAGACTCAAATACAAGA-3’ Reverse: 5’-GACTCAAAGCGACAGATAACACG-3’ |
COX-2 | Forward: 5’-CTGGCGCTCAGCCATACAG-3’ Reverse: 5’-CGCACTTATACTGGTCAAATCCC-3’ |
β-actin | Forward: 5’-TCAAGAAGGTGGTGAAGCAGG-3’ Reverse: 5’-AGCGTCAAAGGTGGAGGAGTG-3’ |