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Fig. 10 | BMC Complementary Medicine and Therapies

Fig. 10

From: Larrea divaricata: anti-inflammatory and antioxidant effects on macrophages and low density lipoproteins

Fig. 10

Effect of AE and NDGA on the structure of HDL subjected to nitrosativestress. HDL was incubated either alone or with 20 µM SIN-1 in either the presence or absence of AE (0.1 µg/ml and 1 µg/ml) or NDGA (0.0003 µg/ml and 0.003 µg/ml).For detection of ApoA1, an HRP-conjugated antibody (anti-Apo-A1 (HRP), 1:1,000 in 10% FCS) was used. Blots were then incubated with the ECL PLUS reagent (Amersham), developed and scanned in a chemiluminescent scanner (LICOR C-DIG it, LICOR Biosciences) and quantified with the Image Studio software. Representative westernblot of fractionated samples.To improve clarity the the cropped blots were used in the figure. The samples of fractionations were derived from the same experiment and the blots were run in parallel.Full-length blot is shown in Supplementary file

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