Fig. 3

CF activated the Nrf2 pathway in the kidney of SAMP8 mice. Scale bars: 50 μm. a The localization of p-Nrf2, p-c-Fos in the kidney was detected by immunohistochemistry. b-e Protein strip and quantification of p-Nrf2, Keap1, and p-c-Fos in the kidney were detected by western blotting. Arrows indicate positive expression. Con: SAMR1 mice; M: SAMP8 mice; CF-L: Treated with low-dose CF ethanol extract; CF-M: Treated with medium-dose CF ethanol extract; CF-H: Treated with high-dose CF ethanol extract; Data represent the mean values ± SD from three independent experiments. ^*P < 0.05 and ^**P < 0.01 vs. M group (n = 3 or n = 6)