Fig. 1

CF improved the aging and fibrosis of the kidneys in SAMP8 mice. a The activity of SA-β-gal in frozen sections of kidneys. Scale bars: 50 μm, magnification 200×. b Changes in the mouse kidney index (kidney index = kidney weight/mouse weight × 100%). c Masson’s trichrome staining of kidneys. Renal fibrosis was expressed by collagen deposition (blue color area) in Masson’s trichrome staining. Scale bars: 50 μm, magnification 400×. d Semi-quantification of renal interstitial fibrosis. The ratio of the blue color area to the field area from Masson’s trichrome-stained sections. e-g The expression levels of Col-I, α-SMA, and FN in kidney tissue were detected by ELISA. Con: SAMR1 mice; M: SAMP8 mice; CF-L: Treated with low-dose CF ethanol extract; CF-M: Treated with medium-dose CF ethanol extract; CF-H: Treated with high-dose CF ethanol extract; Values expressed as mean ± SD; ^*P < 0.05 and ^**P < 0.01 vs. M group (n = 6)