Fig. 2

Origanum essential oil inhibits filamentation of C. albicans and non-albicans strains. Filamentation process was induced in absence and presence of the different treatments at their minimum inhibitory concentration (MIC) obtained previously. Then cells were observed by light microscopy. A Images showing the effect of fluconazole (FCA), nystatin (Nys) and Origanum essential oil (O-EO) on C. albicans ATCC 90029 strain. Arrows indicate the representative cell types observed which each treatment. B Quantification of filamentous cells. Bars represent the mean + SD of the percentage of filamentous cells relative to the total number of cells for each strain. *p < 0.1, **p < 0.01, ***p < 0.001, ****p < 0.0001 with respect to control (vehicle, DMSO); ††p < 0.01, ††††p < 0.0001 with respect to the indicated bar (two-way ANOVA). FCA: fluconazole, Nys: nystatin, O-EO: Origanum essential oil