Table 1 CYP450 isoforms tested with marker reactions at the specific incubation conditions, and Km used in the inhibition study
From: In vitro evaluation of the inhibition potential of echinacoside on human cytochrome P450 isozymes
CYPs | Reaction markers | Concentration of substrate (μM) | Concentration of protein (mg/mL) | Incubation time (min) | Estimated Km (μM) |
|---|---|---|---|---|---|
1A2 | phenacetin O-deethylation | 40 | 0.2 | 30 | 48 |
2E1 | chlorzoxazone 6-hydroxylation | 120 | 0.4 | 30 | 126 |
2C19 | S-Mephenytoin 4-hydroxylation | 100 | 0.2 | 40 | 105 |
3A4 | testosterone 6β-hydroxylation | 50 | 0.5 | 10 | 53 |
2A6 | coumarin 7-hydroxylation | 1.0 | 0.1 | 10 | 1.5 |
2C8 | paclitaxel 6α-hydroxylation | 10 | 0.5 | 30 | 16 |
2C9 | diclofenac 4′-hydroxylation | 10 | 0.3 | 10 | 13 |
2D6 | dextromethorphan O-demethylation | 25 | 0.25 | 20 | 4.8 |