Antibiofilm and anticancer activities of unripe and ripe Azadirachta indica (neem) seed extracts

Table 2 Chemical composition of methanolic extract of unripe neem seed detected by GC-MS analysis

Retention Time	Name of the Compound	% Area	MW (Da)	Molecular formula	Chemical class	Expected FTIR spectra (cm⁻¹)	Biological activities
10.13	4-aminopyrimidine	3.48	95	C₄H₅N₃	Pyrimidine alkaloids	3361, 3169, 1650, 1577, 1563, 1460, 804	Antibacterial activity [76]
13.02	2-oxo-2,3-dihydro-1 H imidazole-4-carbonitrile	1.05	109	C₄H₃N₃0		3456-2778, 1642, 956	Antimicrobial activity and antioxidant activity [77]
17.53	1-napthyl acetoxy acetate	4.61	244	C₁₄H₁₂O₄	Ester compound	1753, 1699, 1378, 1215, 1077, 1045, 777	Antibacterial activity [78]
20.16	4-ethyl-2-hydroxy-2-cyclopentene-1-one	8.65	126	C₇H₁₀O₂	Carbonyl compound	2918, 1705, 1673, 1626, 1436, 1284, 1182, 840	Antibacterial activity [79]
26.14	phthalic acid	11.73	344	C₂₁H₂₈O₄	Aromatic dicarboxylic acid	3094, 3013, 2896, 1701, 1687, 1678, 1405, 1283, 740	Antimicrobial activity [80]
26.55	3,7-dimethyl-(1,2,4)-triazolo-(4,3-b)-(1,2,4)-triazine	3.41	149	C₆H₇N₅	Poly nitrogen containing heterocycles	1571, 1553, 1474, 1401	Anticancer activity [81]
27.24	2-hexyl-tetrahydrothiophane	13.55	172	C₁₀H₂0S	Heterocyclic compound	2963, 2951, 1454, 1440, 1254	Antimicrobial activity [82]

*All the compounds were selected according to the peak area percentage in the GC-MS data. Compounds were cross-checked with their expected FTIR spectra in Fig. 1

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