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Fig. 6 | BMC Complementary Medicine and Therapies

Fig. 6

From: Exploring the total flavones of Abelmoschus manihot against IAV-induced lung inflammation by network pharmacology

Fig. 6

Co-housing with TFA-treated mice confers the protection of IAV-induced lung inflammation. A Diagram of the co-housing experiment. Randomly divided the mice into five groups and administrated with water (Ctrl group), PBS, or TFA (250 mg/kg) for 1 week, separately. Before infected with IAV, TFA-treated mice or PBS-treated mice were reared alone (Single-housing) or co-housed (Co-housing) for 1 week (n = 6 mice per group). B The viral mRNA was examined. The mRNA expression levels of (C) IL-1β, (D) IL-6, (E) TNF-α, (F) IFN-β, (G) Mx2, (H) Oas1, and (I) Oas2 were assessed by real-time PCR. β-actin was used as a control. J The protein and K the total cells in BALF were determined. The proportions of (L) CD11b+Ly6G+ neutrophils and (M) CD11b+F4/80+ macrophages in BALF were detected by flow cytometry. N The pathological changes were evaluated by H&E staining. O Phosphorylated and total levels of p38, ERK1/2, and JNK measured in lung tissue by western blot. The intensities of bands relative to the control were measured with ImageJ software, and the results are shown below. The value are shown as means ± SD of three individual trials. *P < 0.05, **P < 0.01

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