Fig. 4
From: Leaf extract of Osbeckia octandra induces apoptosis in oral squamous cell carcinoma cells
The apoptotic effects of O. octandra leaf extract in OSCC cell. a and b YD10B (a) and HSC2 (b) cells (5 × 105) were grown in 100-mm dishes and incubated at 37 °C overnight. The cells were then treated with 100 μg/ml O. octandra extract for 24 h. Afterwards, the cells were stained with an annexin V-fluorescein isothiocyanate. The annexin V-positive cells (early apoptotic cells) are represented within B4 area in scatter graph (upper panels) and their quantity is indicated by bar graphs (lower panels) (* P < 0.05). c and d YD10B (c) and HSC2 (d) cells were seeded at 1 × 106 cells/100-mm culture dish. After stabilizing for 24 h, the cells were treated with 100 μg/ml O. octandra extract for the indicated time and harvested. Proteins were then extracted from the harvested cell lysates (* P < 0.05). Protein bands were separated on the same gel and were cropped. Cropped blots for Bcl-2, Bcl-XL, Cas-9 (Caspase-9), and C-Cas-3 (Cleaved Caspase3) are shown at 6 h after treatment with 100 μg/ml O. octandra and cropped blots for Bax are shown at 24 h after treatment with 100 μg/ml O. octandra in each OSCC cells. Uncropped blots were shown in Additional file 6: Fig. S6