Fig. 3

The leaf extract of O. octandra induces cell cycle arrest in OSCC cell. a and b YD10B (a) and HSC2 (b) cells were plated at 1 × 10⁶ cells/100-mm culture dish, and then treated with 100 μg/ml O. octandra extract for the indicated time. Propidium iodide staining was performed, and the stained cells were analysed by flow cytometry to evaluate the cell cycle. The histograms for cell cycle analysis are represented at 24 h after treatment with O. octandra extract (upper panels; red line: 0 h skyblue line: 24 h) and their quantity is indicated by bar graphs within cell cycle phase (lower panels) (*** P < 0.001, Mann whitney U test). The details were shown in Table 1. c and d YD10B (c) and HSC2 (d) cells were seeded at 1 × 10⁶ cells/100-mm culture dish. After stabilizing for 24 h, the cells were treated with 100 μg/ml O. octandra extract for the indicated time and harvested. Proteins were then extracted from the harvested cell lysates (* P < 0.05). Protein bands were separated on the same gel and were cropped. Full length of immunoblots was shown in Additional file 5: Fig. S5