Fig. 2
From: Leaf extract of Osbeckia octandra induces apoptosis in oral squamous cell carcinoma cells
The leaf extract of O. octandra interrupts DNA replication in OSCC cell. a and b YD10B (a) and HSC2 (b) cells were plated at 1 × 106 cells/100-mm culture dish. Each well was maintained in the presence or absence of O. octandra (100 μg/ml) for 24 h before carefully adding the BrdU solution directly into the cultured medium. After incubation, BrdU staining was performed. The DNA synthetic activities of cells can be determined by analyzing the correlated expression of total DNA (7-AAD staining) and incorporated BrdU levels. The BrdU-positive cells in S-phase are represented by blue spots (upper panels) and their quantity is indicated by bar graphs (lower panels) (* P < 0.05, Mann whitney U test)