Fig. 5

Effects of Ononin on MAPK and NF-κB signalling pathways. (A) Proteins of p-ERK/ERK, p-JNK/JNK, p-p38/ p38, p-IκBα/IκBα, and p-p65/p65 were detected by Western Blot. The samples derive from the same experiment and that different gels were processed in parallel. (B-F) The expression level of target proteins was expressed by the ratio of grey value of target band to the total of ERK, p38, JNK, IκBα and p65 were used as the internal control respectively by Image-J software. All experiments were performed in 3 times and data were expressed by mean ± SD. ^*P < 0.05 compared with control group; ^#P < 0.05 compared with IL-1β group; ^**P < 0.01 compared with control group; ^##P < 0.01 compared with IL-1β group