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Fig. 3 | BMC Complementary Medicine and Therapies

Fig. 3

From: Panax notoginseng saponins reverse P-gp-mediated steroid resistance in lupus: involvement in the suppression of the SIRT1/FoxO1/MDR1 signalling pathway in lymphocytes

Fig. 3

Effect of PNS on the expression of P-gp and the intracellular accumulation of Rh-123 in SLCs/MP cells. SLCs/MP cells cultured with RPMI-1640 alone are indicated as controls. SLCs/MP cells were incubated with RPMI-1640 alone, PNS (100 μg/ml, 200 μg/ml) or si-SIRT1 for 72 h. Then, the P-gp expression and the efflux pump function were determined by flow cytometry. (A)(F) SLCs/MP cells treated with RPMI-1640 alone. (B)(G) SLCs/MP cells treated with 100 μg/ml PNS. (C)(H) SLCs/MP cells treated with 200 μg/ml PNS. (D)(I) SLCs/MP cells treated with si-SIRT1. (E) Analysis of P-gp expression. (J) Analysis of intracellular accumulation of Rh-123. Experiments were repeated three times and data are expressed as the means ± SD. *P < 0.05 vs. control

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