Fig. 2

Effect of PNS on MP-induced cell apoptosis and CD69 expression. SLCs/MP cells treated with ConA and MP are indicated as controls. After SLCs/MP cells were treated with ConA alone, ConA containing PNS (100 μg/ml, 200 μg/ml), or si-SIRT for 24 h, the cells were cultured with MP (200 μg/ml) for 72 h, and apoptotic cells were measured. (A)(F) SLCs/MP cells treated with ConA. (B)(G) SLCs/MP cells treated with ConA containing 100 μg/ml PNS. (C)(H) SLCs/MP cells treated with ConA containing 200 μg/ml PNS. (D)(I) SLCs/MP cells treated with ConA containing si-SIRT1. (E)(J) Analysis of cell apoptosis and CD69 expression. Experiments were repeated three times and data are expressed as means ± SD. *P < 0.05 vs. control