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Table 1 The compounds identified from the 95% ethanol extracts from B. striata tubers and fibrous roots by UPLC-Q-TOF-MS/MS, and their relative peak areas

From: Discovery and identification of potential anti-melanogenic active constituents of Bletilla striata by zebrafish model and molecular docking

NO.

TR (min)

Formula

Found Mass

[M + H]−

(Da)

Error (ppm)

Identification

Relative peak areas (× 106)

ETB

EFB

1

2.35

C13H18O7

285.0977

161.0450, 123.0444, 105.0352

−0.8

gastrodin

2.17

1.43

2

4.53

C7H6O3

137.0248

137.0244, 108.0224, 92.0275

3.1

p-hydroxybenzoic acid

–

0.77

3

6.70

C9H8O3

163.0406

145.8896, 119.0505, 93.0361

3.2

3-hydroxycinnamic acid

–

0.43

4

7.01

C34H32O8

567.2028

457.1678, 393.1388, 285.0978, 161.0454, 153.0573, 129.0579

0.6

bleochranol D

–

2.80

5

7.71

C40H56O22

887.3179

707.2520, 619.2221, 439.1590

−1.3

dactylorhin A

3.93

3.06

6

8.13

C21H22O8

401.1228

284.0313, 255.0290, 238.0630, 227.0337, 195.0465

−3.6

2,7-dihydroxy-4-methoxyphenanthrene-2-O-glucoside

–

–

7

8.21

C27H32O13

563.1747

540.6504, 429.8704, 394.1452, 320.7833, 310.8648

−4.1

2,7-dihydroxy-4-methoxyphenanthrene-2,7-O-diglucoside

–

1.18

8

9.62

C34H46O17

725.2635

457.1673, 285.0954, 171.0654, 153.0551, 123.0451

−3.8

militarine

30.48

38.39

9

9.76

C20H22O6

357.1332

313.0688, 225.0513, 181.0614, 121.0287, 77.0403

−3.3

pinoresinol

–

–

10

10.31

C21H26O8

405.1537

243.1015, 227.0705, 201.0893, 136.0531, 122.0400

−4.5

3′-hydroxy-5-methoxybibenzyl-3-O-β-D-glucopyranoside

0.72

1.56

11

11.08

C16H12O5

283.0601

237.0654, 211.0864, 75.0467

−3.8

1,8-dihydroxy-3-methoxy-6-methylanthracene-9,10-dione

–

1.53

12

12.15

C15H13O4

256.0734

239.0345, 211.0401, 201.8356, 167.0496, 143.0501

−3.0

7-hydroxy-2-methoxyphenanthrene-3,4-dione

–

–

13

12.42

C15H12O3

239.0714

224.0466, 196.0523, 167.0491

0.3

4-methoxyphenanthrene-2,7-diol

–

1.92

14

12.99

C16H14O4

269.0820

254.0563, 211.0388, 183.0448

0.2

2,7-dihydroxy-3,4-dimethoxyphenanthrene

–

6.01

15

13.54

C51H64O24

1059.3674

791.2752, 661.2301, 569.1989, 439.1569, 153.0557

−3.8

gymnoside X

1.07

0.89

16

13.64

C28H26O5

441.1691

347.1255, 253.0852, 241.0868, 211.0751, 93.0360

−3.7

shanciguol

–

1.51

17

13.80

C16H18O4

273.1126

243.1036, 227.0700, 185.0636, 136.0516, 122.0372, 106.0415

−2.2

3,3′-dihydroxy-5,4′-dimethoxybibenzyl

–

–

18

13.88

C15H16O3

243.1026

227.0707, 183.0810, 136.0537, 93.0357

−0.4

3,3′-dihydroxy-5-methoxybibenzyl

–

1.80

19

14.07

C22H20O4

347.1278

332.1024, 237.0561, 225.0549, 209.0622, 93.0352

−3.2

4,7-dihydroxy-1-(p-hydroxybenzyl)-2-methoxy-9,10-dihydrophenanthrene

0.32

–

20

14.26

C29H24O5

451.1507

437.1341, 392.1347, 329.0777, 224.0484

−9.8

1,8-bis(p-hydroxybenzyl)-4-methoxyphenanthrene-2,7-diol

–

8.73

21

14.31

C22H18O4

345.1124

330.0875, 302.0909, 237.0545

−2.4

1-(p-hydroxybenzyl)-4-methoxyphenanthrene-2,7-diol

0.25

–

22

14.50

C25H24O6

419.1480

405.1118, 377.1172, 225.0548

−4.8

bleochranol B

0.21

1.89

23

14.67

C23H20O5

375.1225

360.0979, 317.0802, 224.0484

−3.6

bleformin B

–

5.08

24

14.71

C30H26O6

481.1631

465.1301, 434.1102, 419.1264, 225.0548

−5.3

blestrin A

0.79

5.08

25

14.89

C22H22O4

349.1434

243.1017, 227.0704, 183.0810, 93.0356

−3.1

3,3′-dihydroxy-4-(p-hydroxybenzyl)-5-methoxybibenzyl

–

–

26

14.94

C30H24O6

479.1478

464.1236, 432.0980, 421.1041, 379.0948, 238.0631, 224.0478, 196.0533

−4.6

blestrin D

0.46

1.91

27

15.53

C32H26O8

537.1529

522.1312, 507.1027, 492.0773, 464.0849, 421.0668, 377.0799, 209.8293, 130.9680

−4.9

4,8,4′,8′-tetramethoxy-[1,1′-biphenanthrene]-2,7,2′,7′-tetrol

0.01

9.15

28

15.74

C37H32O7

587.2047

571.1716, 543.1831, 527.1544, 476.1243, 449.1367, 434.1257, 270.2400, 224.0534, 152.9984

−4.9

bleformin D

–

9.63

29

16.08

C15H14O3

241.0869

226.0615, 183.0449, 169.0667

−0.7

2,4-dimethoxyphenanthrene-7-ol

0.13

1.48

30

16.49

C27H26O7

461.1584

425.1723, 381.1463, 242.0897, 93.0338

−4.7

pleionesin C

0.04

0.36

31

16.61

C16H14O3

253.0862

238.0621, 223.0388, 195.0441, 167.0502

−3.1

2-hydroxy-4,7-dimethoxyphenanthrene

0.01

1.42

32

16.89

C18H18O4

297.1126

253.0491, 239.0346, 225.0942, 211.0392, 166.0456

−2.1

2,3,4,7-tetramethoxyphenanthrene

0.01

2.28

33

16.94

C29H26O5

453.1671

438.1428, 345.1106, 251.0712, 195.0470, 93.0362

−8.2

2,7-dihydroxy-1,6-bis(p-hydroxybenzyl)-4-methoxy-9,10-dihydrophenanthrene

0.10

0.64

34

17.46

C23H24O4

363.1585

333.1163, 255.0940, 227.0706, 199.0746, 157.0631, 93.0378

−4.7

bulbocol

0.15

3.85

35

17.67

C22H22O3

333.1486

163.1126, 107.0521, 75.0477

−3.2

5-hydroxy-2-(p-hydroxybenzyl)-3-methoxybibenzyl

0.60

3.34

36

18.27

C27H35O9

502.2211

369.2416, 337.2141, 193.1229, 163.1124, 147.0816, 133.1056

0.5

(20S,22R)-1β,2β,3β,4β,5β,7α-hexahydroxyspirost-25 (27)-en-6-one

9.14

1.06

37

18.30

C18H30O3

293.2117

275.1976, 249.1821, 235.1714, 221.1501, 171.1030, 121.1041, 59.0153

−1.7

striatolide

–

2.49

38

18.78

C16H32O2

255.2327

238.027, 224.0464, 210.0314, 195.0444, 182.0355, 167.0502

−1.0

palmitic acid

–

0.95

39

18.87

C31H23O8

522.1329

508.1470, 493.1272, 450.1078, 253.0495, 225.0527, 210.0426

1.7

3′,7′,7-trihydroxy-2,2′,4′-trimethoxy-[1,8′-biphenanthrene]-3,4-dione

7.20

0.76

Total

      

57.79

123.37

  1. - means the relative peak areas is less than 0.01 × 106