Fig. 5

HG-induced BMDM aggravated the inflammatory response of MES cells. a, The schematic diagram of the co-culture system. b-d, Effect of A&P on the mRNA expression of TNF-α, IL-1β and IL-6 in HG-induced MES cells and co-culture cells detected by Real-time PCR analysis. e-i, Effect of A&P on the protein level of p-NFκB, TNF-α, IL-1β and IL-6 in HG-induced MES cells and co-culture cells detected by western blotting analysis. (The data are expressed as the mean ± SD. Compared with Control group of single culture, *P < 0.01. Compared with HG group of single culture, ^#P < 0.01. Compared with HG group of single culture, **P < 0.01. Compared with HG group of co-culture, ^#P < 0.01)