Fig. 4

A&P inhibits inflammaion in BMDM cells. a-b, MTT viability assay. c-f, Effect of A&P on the mRNA expression of Mincle, TNF-α, IL-1β and IL-6 in HG-induced BMDM cells according to Real-time PCR analysis. g-i, the content of TNF-α, IL-1β and IL-6 in supernatant in HG-induced BMDM cells detected by ELISA assay. j, The relative expression of p-NFκB detected by western boltting analysis normalized to NFκB expression, and the relative expression of Mincle, Card9, TNF-α, IL-1β and IL-6 detected by western boltting analysis normalized to β-actin expression. (The data are expressed as the mean ± SD. Compared with Control group, *P < 0.05. Compared with HG group, ^#P < 0.05, ^##P < 0.01)