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Fig. 5 | BMC Complementary Medicine and Therapies

Fig. 5

From: Protective effects of exosomes derived from lyophilized porcine liver against acetaminophen damage on HepG2 cells

Fig. 5

Live imaging of oxidative stress metabolic markers. Microphotographs taken stitching 4 images of HepG2 cells cultured on a 48-well plate of a live imaging immunofluorescence, representative for 3 experiments. Without APAP (panel A and D): no staining (Null), control cells (CTRL), TEV- and EEV-treated cells. With APAP (panel B and E): vehicle-treated cells (DMSO), control cells (CTRL), TEV- and EEV-treated cells. (A,B) CellROX staining (red) on HepG2 cells showing level of hydrogen peroxides in the cytoplasm (image size: 1560 × 1560 μm). C Diagram showing number of CellROX positive cells in CTRL, TEV and EEV-treated cells with or without 24 h APAP (or vehicle) treatment. After fluorescence background subtraction, positive cells in the microphotographs are counted. D, E MitoSOX staining (red) on HepG2 cells. MitoTracker counterstaining (green) showing basal mitochondrial ROS activity (image size: 1145 × 1145 μm). (F) Diagram showing fluorescence levels of MitoSOX positive cells in CTRL, TEV and EEV-treated cells with 24 h APAP (or vehicle) treatment. After fluorescence background subtraction, MitoSOX fluorescence levels were evaluated in positive counted cells. For all panels, data are mean ± SD with n = 3 per group. *, p < 0.05 vs CTRL; Φ, p < 0.05 vs APAP treatment; #, p < 0.05 vs vehicle (DMSO)

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