Fig. 3

Evaluation of metabolic marker proteins: TEV and EEV treatments differently affected protein expression after APAP administration. Diagrams showing densitometric analysis of protein levels (A, c-Casp3; B, c-PARP; C, p-c-Jun; D, p-JNK; E, BiP) in HepG2 APAP-treated cells, vehicle-treated cells (DMSO) and APAP plus TEV or EEV pretreatments. All protein levels are normalized to the total protein expression of each sample measured using the TGX stain-free technology by Bio-Rad (F). For all panels, data are mean ± SD with n = 3 per group. *, p < 0.05 vs DMSO (control); Φ, p < 0.05 vs APAP. (Shown lines were cropped from the original row file. Full-length blots are displayed in (Additional file 5)