Table 1 Primers and Probe Sequences for Real-time PCR
Gene | Primers and Probes | Sequence (5′ → 3′) |
|---|---|---|
IL-6 | Forward | CGGGAACGAAAGAGAAGCTCTA |
Reverse | CGCTTGTGGAGAAGGAGTTCA | |
Probe | TCCCCTCCAGGAGCCCAGCT | |
TNF-α | Forward | AACATCCAACCTTCCCAAACG |
Reverse | GACCCTAAGCCCCCAATTCTC | |
Probe | CCCCCTCCTTCAGACACCCTCAACC | |
COX-2 | Forward | GAATCATTCACCAGGCAAATTG |
Reverse | TTTCTGTACTGCGGGTGGAAC | |
Probe | TTCCTACCACCAGCAACCCTGCCA | |
GAPDH | Forward | GAAGGTGAAGGTCGGAGTC |
Reverse | GAAGATGGTGATGGGATTTC | |
Probe | CAAGCTTCCCGTTCTCAGCC |