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Fig. 4 | BMC Complementary Medicine and Therapies

Fig. 4

From: Anti-inflammatory effect of lavender (Lavandula angustifolia Mill.) essential oil prepared during different plant phenophases on THP-1 macrophages

Fig. 4

Effects of linalool and LEOs and NFκB inhibitors pretreatments on mRNA (A,C,E,G) and protein (B,D,F,H) levels of pro-inflammatory cytokines IL-6, IL-8, IL-1β and TNFα. THP-1 cells were pretreated with 500-fold diluted linalool, LEOs and 5 μM luteolin or ACHP for 24 h then 100 ng/mL P. aeruginosa LPS was added to the pretreated cells for 24 h. DMSO administration was used as a control of the treated cells. Real Time PCR was performed with SYBR green protocol using specific primers. β-actin was used as housekeeping gene for the normalization and the relative expression of controls was considered as 1. Pro-inflammatory cytokine secretions were determined using IL-6, IL-8, IL-1β and TNFα specific ELISA kits according to the manufacturer’s instructions. The bars represent mean values and error bars represent standard deviation (SD) for three independent determinations (n = 3). Real Time PCR and ELISA measurements were carried out in triplicate in each independent experiment. Asterisks indicate p < 0.05 compared to the control. Crosses show p < 0.05 compared to the LPS treatment. Double cross represents p < 0.05 compared to luteolin treatment. Number sign marks p < 0.05 compared to ACHP treatment. The diagrams were created using the same logical structure and the different treatments appeared in the same sequence in each figure in order to improve traceability of the results

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