Table 1 Primer sequence design for Real Time PCR assessment
Accession number | Gene | Primer sequence | Product length (Bp) | Melt Temperature (°C) |
|---|---|---|---|---|
XM_006234398.3 | Nrf2 | F 5′ GAAAACGACAGAAACCTCCATC 3′ R 5′ CTCCATCCTCCCGAACCT 3′ | 202 | 83 |
XM_017593963.1 | GAPDH | F 5′ CAT ACT CAG CAC CAG CAT CAC C 3′ R 5′ AAG TTC AAC GGC ACA GTC AAG G 3’ | 121 | 79.65 |