Fig. 4

Anti-apoptosis effect of Etpost (2 μg/ml) on the isolated sections of rat heart tissue. Anti-apoptosis effect of ethyl acetate fraction from P. reptans on the isolated sections of rat heart tissue. The effect of IR, L-NAME, Etpost (2 μg/ml) and Etpost (2 μg/ml) + L-NAME on apoptotic markers. A Expression of BAX and BCl-2 proteins after nuclei staining with DAPI and obtained from fluorescence microscope (observed under 400 magnifications). B Expression of the levels of caspase-3 protein. C Representative TUNEL stained and captured by fluorescence microscopy of rat heart sections with IR and Etpost (2 μg/ml) groups. The green nuclear structures represent apoptotic cells. The nuclei are stained by PI. The data were expressed as mean ± SEM. *P < 0.05 vs. IR group. Etpost: ethyl acetate fraction of P. reptans root