Fig. 4

eGSM decreased the phosphorylated mTOR in SNU2292, not in A549. A549 cells (A) or SNU2292 cells (C) were treated with indicated amounts of eGSM for 16 h (lanes 2 to 5), along with rapamycin (10 μM, 6 h). Total proteins were extracted and measured by Western blotting for mTOR phosphorylated at S2448. The blotted membrane was stripped and reprobed for mTOR to ensure equal loading. The bands were analyzed by ImageJ and the relative levels of the phosphorylated mTOR over mTOR in A549 (B) and SNU2292 (D) are shown. Three measurements of each band are presented as in the mean ± SEM. *P was less than 0.05, compared to untreated controls