Fig. 5
From: Ethyl acetate extract of Elephantopus mollis Kunth induces apoptosis in human gastric cancer cells
The caspase-3 activation of AGS cells treated with EM-EA extract. a AGS control cells treated with 0.1% (v/v) DMSO at 48 h. b AGS cells treated with EM-EA extract (27.5 μg/ml) at 48 h. c AGS cells treated with doxorubicin (0.3 μg/ml) at 48 h. d The percentage of activated-caspase 3-expressing cells of control samples (n = 3), EM-EA-treated samples (n = 3), and DOX-treated samples (n = 2) at 48 h. Each value represents mean ± SD. The statistical difference was analyzed by two-tailed unpaired Student’s t-tests (****p < 0.0001). Columns (1), (2), (3), (4) represent images of cell morphology (bright field), nuclear morphology (blue), activated-caspase-3 (green), and merging of (2) and (3) (blue and green). Arrowheads, arrows, and thin arrows point abberant cell morphology, nuclear fragmentation, and caspase-3 activation respectively