Fig. 2

Effects of HDR-2 on the expression of NOS isoforms at the mRNA level (as assessed by RT-PCR). a RT-PCR analysis of iNOS mRNA expression in murine RAW 264.7 macrophages. Activated cultures were stimulated for 6 h with 1 μg/mL LPS or individual extracts before extraction of RNA. b RT-PCR analysis of nNOS mRNA expression in cultured rat hippocampal neurons. Activated cultures were stimulated for 6 h with 50 μM _L-glutamate (Glu) or individual extracts before extraction of RNA. c RT-PCR analysis of eNOS mRNA expression in HUVECs. Activated cultures were stimulated for 6 h with 10 μM ACh or individual extracts before extraction of RNA. d eNOS mRNA expression was detected by real-time quantitative RT-PCR. ^*/#P < 0.05, ^**/##P < 0.01 and ^***/###P < 0.001 vs the CTL group; NS, not significant. Symbols refer to significant differences (* symbol: increase/# symbol: decrease) between the treated group and the control group