Fig. 5

The effects of the three active ingredients on the differentiation of APP-NSCs. APP-NSCs were cultured in differentiation medium, exposed to DISS (60 μM); OB (10 μM), and TEN (60 μM) for 7 days, then the cell types were detected using by IF analysis against GFAP, NF-M and NG2 antibody, respectively. (A) The representative micrographs of APP-NSCs differentiated into astrocytes (GFAP⁺); neuronal cells (NF-M⁺) and oligodendrocytes (NG2⁺). Scale bar = 100 μm; (B) The quantitative analysis of astrocytes, neurons, and oligodendrocytes as in A. #P < 0.05, ##P < 0.01 vs. GFP-NSCs group; *P < 0.05; **P < 0.01 vs. APP-NSCs group. Data are represented as mean ± sem. This experiment was performed three times. Data were analyzed by one-way ANOVA