Fig. 3

Tan IIA alleviated apoptosis. (A) Representative images of apoptotic cells in the renal tissue, as analyzed by the TUNEL assay, Scale bar: 50 μm. (B) Statistics analysis showed that fewer apoptotic cell in renal tissue of control group and Tan IIA groups at 0 h, however, 24 h and 48 h of hypothermic preservation triggered an increase in the number of apoptotic cells (arrow), whereas Tan IIA reduced the number of apoptotic cells, *P < 0.05; **P < 0.01; ***P < 0.001; n = 5. (C) The expression of cleaved caspase-3 was reduced by Tan IIA. ns > 0.05; *P < 0.05; **P < 0.01; ***P < 0.001; n = 3