Table 1 Sequences of primers used in the study
Gene | Sequence (5′ -3′) | Size |
|---|---|---|
miR-625-5p-Forward | ACTCCAGCTGGGAGGGGGAAAGTTCTATAG | 71 bp |
miR-625-5p-Reverse | CTCAACTGGTGTCGTGGA | |
U6- Forward | CTCGCTTCGGCAGCACA | 96 bp |
U6- Reverse | AACGCTTCACGAATTTGCGT | |
BMP2-Forward | ACGCCTTAAGTCCAGCTGTA | 160 bp |
BMP2-Reverse | GGCATGATTAGTGGAGTTCA | |
Runx2- Forward | TCTAAATCGCCAGGCTTCAT | 250 bp |
Runx2-Reverse | GAGGACCTACTCCCAAAGGA | |
Osteocalcin- Forward | CTCACACTCCTCGCCCTATT | 139 bp |
Osteocalcin-Reverse | TGGGTCTCTTCACTACCTCG | |
H19-Forward | GCGGGTCTGTTTCTTTACTTC | 171 bp |
H19-Reverse | GTGGTTGTAAAGTGCAGCAT | |
18s-Forward | CCTGGATACCGCAGCTAGGA | 112 bp |
18s-Reverse | GCGGCGCAATACGAATGCCCC |