Fig. 6

miR-625-5p overexpression reversed quercetin effects on BMSCs. A miR-625-5p expression was measured by qRT-PCR at 24 h after transfection. B Proliferation of transfected BMSCs was measured using the Cell Counting Kit-8 on days 1, 2, and 7 following quercetin treatment. C ALP activity was measured using the Alkaline Phosphatase Assay Kit, and BMP-2, RUNX2, and osteocalcin mRNA expressions were measured by qRT-PCR after transfection and quercetin treatment for 21 days. D Calcium nodule formation was detected using Alizarin Red staining (400×) after transfection and quercetin treatment for 21 days. *P < 0.05 and ***P < 0.001