Fig. 5

H19 interacts with miR-625-5p and inhibits miR-625-5p expression. A miR-483-3p and miR-625-5p were selected from the intersection of the GSE148049 dataset, Starbase 3.0, and LncBase Experimental v.2 analysis results. Differentially expressed miRNAs during BMSC osteogenic differentiation were identified from by the GSE148049 dataset. Potential miRNAs binding to H19 were determined according to Starbase 3.0 and LncBase Experimental v.2 analysis. B miR-483-3p and miR-625-5p expression were analyzed in the GSE148049 dataset. ***P < 0.001 vs Day 0. C The binding sites between H19 and miR-625-5p were analyzed by Starbase 3.0. D The binding of H19 to miR-625-5p was measured by the luciferase reporter assay. ***P < 0.001. E miR-625-5p expression reduced during quercetin-induced BMSC osteogenic differentiation. **P < 0.01 and ***P < 0.001, vs Blank group. F miR-625-5p expression negatively correlated with H19 expression. G miR-625-5p expression increased at 21 days after transfection. ***P < 0.001