Table 1 The sequence of primers used for gene expression
Gene Name | Sequences | Accession No. | No. of first and last Nt. | Product length |
|---|---|---|---|---|
GAPDH-FW | 5′-CCTGTGACTTCAACAGCAACTC-3´ | NM_017008.4 | 917–938 | 177 bp |
GAPDH-RW | 5′-GGTGGTCCAGGGTTTCTTACTC-3´ | 1072–1093 | ||
PPAR-γ-FW | 5′-GTTCACAAGAGCTGACCCAATG-3´ | NM_013124.3 | 332–353 | 135 bp |
PPAR-γ-RW | 5′-TGTGGCCTGTTGTAGAGTTGG-3´ | 446–466 | ||
TNF-α-FW | 5′-CCAAATGGGCTCCCTCTCATC-3´ | NM_012675.3 | 337–357 | 112 bp |
TNF-α-RW | 5′-CCGCTTGGTGGTTTGCTAC-3´ | 430–448 | ||
IL-10-FW | 5′-GCGACGCTGTCATCGATTTC-3´ | NM_012854.2 | 370–389 | 153 bp |
IL-10-RW | 5′-AGTGTCACGTAGGCTTCTATG C-3´ | 501–522 |