Fig. 3

The demonstration of ROCEN allergenicity evaluation using MEST. A Swelling measurement of the left ear of the mice did not show any changes via ROCEN application. n = 10 for all groups (^# P< 0.0001, formaldehyde group compared to control group). B The induction stage through intradermal injections of the relative substances on the mice abdomen that were applied topically by rubbing gently. The lesion site was observable in the formaldehyde group on the 5th day of the experiment. C The representative mice ear shows a considerable ear redness in the formaldehyde group (sensitized mice) but without erythema or swelling in mice receiving ROCEN on the 12th day