Fig. 5

Ruyiping inhibits breast cancer metastasis in vivo. Mice transplanted with 4 T1 cells (n = 5) and gavage administration once a day with RYP. Xenograft tumor growth was photographed and calculated by the formula V = (W)² × L/2 (A and B). Metastatic nodules in lung were stained with Bouin’s solution and counted (C-E). SLUG, E-Cadherin, N-Cadherin and pre-miR-134 expression in primary tumors were detected by real-time PCR (F-I) or western blot (J). (E) HE staining of lungs five weeks after injection of 4 T1 cells and treated with saline or RYP. Scale bars = 0.5 mm. Arrows designate region of 200× magnification shown in insets. Scale bars = 0.1 mm. (K) Immunohistochemical analysis of SLUG in primary tumors. Scale bars = 0.01 mm. *P < 0.05, **P < 0.01. P-values were determined by one-way analysis of variance. Data are mean ± standard error of the mean and are representative of three independent experiments