Fig. 2From: Ruyiping formula inhibits metastasis via the microRNA-134-SLUG axis in breast cancerRuyiping inhibits SLUG gene expression through miR-134 induction. Potential target miRNAs of SLUG 3’UTR were co predicted with the TargetScan, miRWalk and miRDB databases (A). Pre-miR-134 in 4 T1 cells stimulated with different dose of RYP (B) for 24 h, 10 μg/mL RYP (C) for indicated times. SLUG production in MDA-MB-231 and 4 T1 cells were detected after transfected with miR-22, miR-134 chemically synthesized mimics (D) or inhibitors (E) or treated with RYP (F and G). *P < 0.05, **P < 0.01, and ***P < 0.001. P-values were determined by one-way analysis of variance. Data are mean ± standard error of the mean and are representative of three independent experimentsBack to article page