Table 1 Real-time PCR primer sequence
From: Effects of Stephania hainanensis alkaloids on MSU-induced acute gouty arthritis in mice
Gene | Forward primer (5′ to 3′) | Reverse primer (5′ to 3′) |
|---|---|---|
NLRP3 | GGGACTCAAGCTCCTCTGTG | GAGGCTCTGGTTATGGGTCA |
Caspase-1 | TCCGCGGTTGAATCCTTTTCAGA | ACCACAATTGCTGTGTGTGCGCA |
IL-1훽 | CCTCGTGCTGTCGGACCCATA | CAGGCTTGTGCTCTGCTTGTGA |
TNF-훼 | CAAAGGGAGAGTGGTCAGGT | GGCAACAAGGTAGAGAGGC |
GAPDH | TGTCATACTTGGCAGGTTTCT | CGTGTTCCTACCCCCAATGT |