Fig. 5

NLRP3, Caspase-1, and IL-1β expression in gouty ankle tissue of MSU- induced mice. Protein expression of NLRP3 and substrates in mouse ankle tissue was analysed by Western blotting after MSU crystals suspension (2.5 mg/50 ml) was injected intradermally for 48 h (A). B-D the greyscale values of the bands were quantified by Image-Pro Plus Version 7.0 and GAPDH was used as a loading control. The data shown are the mean ± SD of at least three independent experiments. ^## P < 0.01 versus the saline group; * P < 0.05, ** P < 0.01 versus the MSU group