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Fig. 1 | BMC Complementary Medicine and Therapies

Fig. 1

From: A radiosensitizer, gallotannin-rich extract from Bouea macrophylla seeds, inhibits radiation-induced epithelial-mesenchymal transition in breast cancer cells

Fig. 1

Maprang seed extract (MPSE) enhances the radiosensitivity of breast cancer cells. A MCF7 and MDA-MB231 cells were pre-treated with 20 μg/mL MPSE for 24 h and then subjected to increasing doses of ionizing radiation (IR); the survival fraction of MCF7 and MDA-MB231 cells at 14 days post-treatment were determined by clonogenic assay. B Representative fluorescence images of γH2AX foci. MCF7 and MDA-MB231 cells were grown on glass coverslips and pre-treated with MPSE for 24 h and then irradiated with 6 Gy of IR. After 24 h, cells were analyzed for γH2AX foci by immunostaining with anti-γH2AX. C Quantitative assessment of mean fluorescence intensity values of γH2AX in breast cancer cells. More than 100 cells were randomly picked for quantification. Results are presented as mean ± SD. The statistical significance of each group was analyzed by a one-way ANOVA and Tukey’s HSD test (* p < 0.05.) D Western blotting analysis with anti-γH2AX and anti-GAPDH antibodies on breast cancer cells treated with IR and MPSE, alone or in combination. The immunoblot signal intensities were quantified by densitometry; the mean data from each experiment were normalized to the GAPDH. Three independent experiments were performed. The results are presented as mean ± SD (numbers above the bars represent the mean of each group). The statistical significance of each group was analyzed by a one-way ANOVA and Tukey’s HSD test (* p < 0.05)

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