Phytochemical, acute toxicity and renal protective appraisal of Ajuga parviflora hydromethanolic leaf extract against CCl4 induced renal injury in rats

Table 2 Toxicity profiles of APHM test samples using brine shrimp cytotoxicity test

Groups	Dose μg/mL	Survivors (%)	LC₅₀^c μg/mL	Toxicity profile
DMSO^d (v/v)	1000	100	–	Non-toxic
	5000	100
	10,000	100
	25,000	100
APHM^e	10	100	2853.1	Non-toxic
	100	82 ± 7.64
	1000	71.4 ± 9.33^a
	10,000	22.1 ± 6.24^b
Etoposide	10	19.4 ± 4.25^b	8.4	Toxic

Clarkson criteria: LC₅₀ > 1000 μg/mL are non-toxic, LC₅₀ of 500–1000 μg/mL are low toxic, LC₅₀ of 100–500 μg/mL are medium toxic, LC₅₀ of 0–100 μg/mL are highly toxic
^ap ≤ 0.05 significant as compared to control, ^bp ≤ 0.005 highly significant as compared to DMSO
^cLethal concentration, ^dDimethyl sulfoxide, ^eAjuga parviflora hydromethanolic extract

ISSN: 2662-7671