Fig. 2

Effect of Silibinin on apoptosis and autophagic cell death. A) Representative flow cytometry plots of CT26 cells stained with Propidium iodine/Annexin V-FITC at 24 h after treatment with 50 μM of silibinin. B) Effect of silibinin on the expression of Bax, Bcl-2 and Caspase-3 were detected by RT-qPCR. C) RT-qPCR analysis of autophagy gene expressions in CT26 cells treated 50 μM of silibinin. Data are represented as mean ± standard error of the mean (n = 3). *P < 0.05, **P < 0.01 and ***P < 0.001 versus control group