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Fig. 4 | BMC Complementary Medicine and Therapies

Fig. 4

From: The polyphenol/saponin-rich Rhus tripartita extract has an apoptotic effect on THP-1 cells through the PI3K/AKT/mTOR signaling pathway

Fig. 4

S6 and AKT activation is inhibited by Rhus tripartita extracts. a Immunoblot analysis with antibodies specific for AKT, phosphorylated AKT (pAKT), S6 or phosphorylated S6 (pS6) of THP-1 whole cell extracts before (CTRL) or after treatment with the indicated concentration of the Rhus tripartita total extract (RTE), saponin extracts (SE), rutin (R), kaempferol (K), a mixture of catechin, epicatechin and epicatechin-gallate (CEEG) or ellagic acid (EA) for 48 h. Full-length blots from the two gels used for this figure are shown in Supplementary Fig. 1 (Fig. S1). b The protein levels were quantified, normalized to the γ-tubulin loading control levels and expressed as fold increase relative to CTRL cells. Bars indicate SEM (n = 3). For each treatment, the levels of phosphorylated proteins were compared to the levels of the unphosphorylated form. Asterisks (*), indicates Student’s t-test with p < 0.05 (*), p < 0.01 (**)

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