Fig. 4

Effects of over-expressing CYTOR on the viability, apoptosis and inflammatory injury of chondrocytes. a CYTOR overexpression models of chondrocytes were constructed using overexpression plasmids. b The expression of CYTOR in chondrocytes was detected by qRT-PCR after the chondrocytes were treated with IL-1β. c-d The overexpression plasmid was transfected into chondrocytes, and then the cells were stimulated by IL-1β, and the cell activity was detected by CCK-8 assay. e After the chondrocytes were transfected with CYTOR overexpression plasmid, the cells were stimulated by IL-1β, and apoptosis was detected by flow cytometry. f, g h After the chondrocytes were overexpressed with CYTOR and stimulated by IL-1β, and the levels of IL-6, IL-8 and TNF-α were detected by ELISA. *P < 0.05, **P < 0.01 vs. control group (chondrocytes stimulated by IL-1β)