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Table 1 Real-time PCR gene primer list

From: Three chemotypes of thyme (Thymus vulgaris L.) essential oil and their main compounds affect differently the IL-6 and TNFα cytokine secretions of BV-2 microglia by modulating the NF-κB and C/EBPβ signalling pathways

Primer

Sequence 5′ → 3’

IL-6 forward

CTCTGCAAGAGACTTCCATCCA

IL-6 reverse

GACAGGTCTGTTGGGAGTGG

TNFα forward

GATCGGTCCCCAAAGGGATG

TNFα reverse

CCACTTGGTGGTTTGTGAGTG

β-actin forward

CTGTCGAGTCGCGTCCA

β-actin reverse

TCATCCATGGCGAACTGGTG

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BMC Complementary Medicine and Therapies

ISSN: 2662-7671

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